The effect of diazinon exposure on hepatic tissue and enzymes in male frog Rana sridibunda

Document Type: Research Paper


1 M.S. Payamenoor University, Iran

2 Assistant professor, Department of Biology, Faculty of Science, Islamic Azad University, Arak Branch, Arak, Iran

3 Associate Professor, Biology Department, PayamNoor University,19395-4697 Tehran, Iran

4 Assistant Professor, Cardiac Surgery Department, Arak University of Medical Science, Iran


The purpose of this study was to examine the acute and sub-acute effects of diazinon on
histopathological parameters of liver tissue and blood level of hepatic enzymes of male common
frog (Rana ridibunda). Frogs were assigned to 9 groups (n=8, meaning 8 frogs in each group):
control and treatment groups with 30, 60, 90 and 120 μg/L diazinon in water for one day or one
week. Hepatic enzymes containing alanine transaminase (ALT), aspartate aminotransferase
(AST), and alkaline phosphatase (ALP) were measured using biochemical kit. All ALP values
were significantly different between the control group and one-day and one-week diazinon
exposed groups. Hepatocyte changes were observed only for diazinon concentrations greater
than 60 μl after one week. The most important characteristics of the affected cells were
hyperplasia and chromatin formation. Results show that sub-lethal concentrations of diazinon
have an increasing effect on the blood level of hepatic enzymes, which probably indicated the
detoxification response of liver on the basis of diazinon sub-lethal effect model.


Main Subjects

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