• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Editorial Staff
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter Telegram
Progress in Biological Sciences
Articles in Press
Current Issue
Journal Archive
Volume Volume 6 (2016)
Volume Volume 5 (2015)
Volume Volume 4 (2014)
Volume Volume 3 (2013)
Issue Issue 2
Summer and Autumn 2013, Page 1-156
Issue Issue 1
Winter and Spring 2013, Page 1-117
Volume Volume 2 (2012)
Volume Volume 1 (2011)
Akbarzadeh, A., Ranaei Siadat, S., Zamani, M., Motallebi, M., Barshan Tashnizi, M. (2013). Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha. Progress in Biological Sciences, 3(1), 108-117. doi: 10.22059/pbs.2013.32100
Ali Akbarzadeh; Seyed Omid Ranaei Siadat; Mohammad Reza Zamani; Mostafa Motallebi; Mohammad Barshan Tashnizi. "Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha". Progress in Biological Sciences, 3, 1, 2013, 108-117. doi: 10.22059/pbs.2013.32100
Akbarzadeh, A., Ranaei Siadat, S., Zamani, M., Motallebi, M., Barshan Tashnizi, M. (2013). 'Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha', Progress in Biological Sciences, 3(1), pp. 108-117. doi: 10.22059/pbs.2013.32100
Akbarzadeh, A., Ranaei Siadat, S., Zamani, M., Motallebi, M., Barshan Tashnizi, M. Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha. Progress in Biological Sciences, 2013; 3(1): 108-117. doi: 10.22059/pbs.2013.32100

Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha

Article 15, Volume 3, Issue 1, Winter and Spring 2013, Page 108-117  XML PDF (949 K)
Document Type: Research Paper
DOI: 10.22059/pbs.2013.32100
Authors
Ali Akbarzadeh1; Seyed Omid Ranaei Siadat* 2; Mohammad Reza Zamani3; Mostafa Motallebi3; Mohammad Barshan Tashnizi4
1Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB); Nanobiotechnology Engineering Laboratory, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, GC,
2Nanobiotechnology Engineering Laboratory, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, GC, Tehran, Islamic Republic of Iran
3Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Islamic Republic of Iran
4Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Islamic Republic of Iran
Abstract
Bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. One of the most important organisms that produce cellulases is the filamentous fungi, Trichoderma reesei which able to secrete large amounts of different cellulases. These enzymes are probably the most widely used cellulases industrially, however, the cellulases excreted from fungi are not stable at high pH or high temperatures. In this study methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha were used for the comparative heterologous production of endoglucanase II. Two synthetic egII genes with P. pastoris and H. polymorpha codon preferences were transferred into the yeasts. In addition, both expression vectors contained the pre-pro-sequence of Saccharomyces cerevisiae mating factor alpha to allow secretion of protein. Enzymes characterization demonstrated increasing thermal stability in both recombinants EGII compare with native enzyme from T. reesei and the Hansenula enzyme was more stable than Pichia in higher temperature. Biochemical properties determination on different substrates showed higher binding site affinity in Pichia than Hansenula and native one. We can conclude that P. pastoris and H. polymorpha are appropriate hosts for expression and production of endoglucanase with improved thermal stability.
Keywords
Hansenula polymorpha; Pichia pastoris; recombinant protein; endoglucanase
Statistics
Article View: 2,723
PDF Download: 3,944
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by sinaweb.