Effects of soil conductivity on properties of saffron corms and in vitro production of its style explants

Document Type: Research Paper


Faculty of Science, Alzahra University, Tehran, Iran, P.O. Box: 1993891176


Saffron is the dried stigmas of Crocus sativus L., a member of the Iridaceae family which is
propagated by means of corms. Corms are faced with many stresses in soil. Therefore, it is
important to reduce these stresses and improve the quantity of saffron production. Biotic and
abiotic stresses disrupt the metabolic balance of cells; thereby, resulting in accumulation of
reactive oxygen species (ROS) which cause oxidative damage. In this study, the effect of soil
electrical conductivity (EC) on biochemical indicators of corms, the percentages of callus
formation and stigma-like structures (SLSs) on calli were investigated. In order to obtain calli
and SLSs, immature style explants from floral buds of corms were collected from three regions
(Shahroud, Mardabad and Torbat Heydarieh) and used for tissue culture. Style explants were
separated first from the immature floral buds, then sterilized and used for tissue culture.
Biochemical analysis of calli with SLSs including malondialdehyde (MDA) and proline
contents, antioxidant enzymes activities and polysaccharides and reducing sugars contents were
investigated and compared. Moreover, sodium and potassium ions content and EC of soils of
the three regions were investigated. The results indicated that corms from Shahroud with the
highest level of EC soil showed more imposed stress than that from Torbat Heidariye and
Mardabad but the calli percentage and number of SLSs of Mardabad’s were higher than those of
the other two respectively. In this study, a close relationship between soil EC and in vitro
production of saffron with a short glance on epigenetic modification was postulated.


Main Subjects

1. Kafi, M., Koocheki, A., Rashed, M.H., Nassiri, M. (eds.) (2006). Saffron (Crocus sativus)
Production and Processing (1st ed.), Sci. Publishers, ISBN 978-1-57808-427-2.
2. Carillo, P., Annunziata, M. G., Pontecorvo, G., Fuggi, A., & Woodrow, P. (2011). Salinity stress
and salt tolerance. Abiotic Stress Plants-Mech. Adapt.
3. Finkel, T. (2011). Signal transduction by reactive oxygen species. J. cell boil. 194, 7-15.
4. Graves,D.B. (2012). The emerging role of reactive oxygen and nitrogen species in redox biology
and some implications for plasma applications to medicine and biology. J. Phys. D Appl. Phys,
5. Chiang, H.H. and Dandekar, A.M. (1995). Regulation of proline accumulation in Arabidopsis
thaliana (L.) Heynh during development and in response to dessication. Plant Cell Environ 18,
6. Moustakas, M., Sperdouli, I., Kouna, T., Antonopoulou, CI. and Therios, I. (2011). Exogenous
proline induces soluble sugar accumulation and alleviates drought stress effects on photosystem II
functioning of Arabidopsis thaliana leaves. Plant Growth Regul.65:315–25.
7. Pérez-López, U., Robredo, A., Lacuesta, M., Muñoz-Rueda, A. and Mena-Petite, A. (2010).
Atmospheric CO2 concentration influences the contributions of osmolytes accumulation and cell
wall elasticity to salt tolerance in barley cultivars. J. Plant Physiol. 167, pp. 15–22.
8. Miller, G., Suzuki, N., Ciftci-Yilma, S. and Mittler, R. (2010). Reactive oxygen species
homeostasis and signaling during drought and salinity stresses. Plant Cell Environ. 33, pp. 453–
9. Sarma, KS., Maesato, K., Hara, T. and Sonida, Y. (1990). In vitro production of stigma-like
structures from stigma explants of Crocus sativus L. J. Exp. Bot. 41,745–748.
10. Hosseinzadeh Namin, M., Ebrahimzadeh, H., Ghareyazie, B., Radjabian, T., Gharavi, S. and
Tafreshi, N. (2010). Initiation and origin of stigma-like-structures (SLS) on ovary and style
explants of saffron in tissue culture. Acta. Biol. Cracov. Bot. 52, 1,55–60.
11. Ganai, MY. (2002). Corm root disease of saffron and its management. In proceeding of seminarcum-
workshop on saffron (Crocus sativus). Skuast-k, India, 107-112.
12. Paseban, M. and Rezaian, S. (2006). The Effect of Micronutrients and Manure Fertilizers on the
Quantity and Quality of Khorasan Saffron. In II International Symposium on Saffron Biology and
Technology 739 (pp. 155-158).
13. Murashing, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with
tobacco tissue culture. Plant physiol. 15,473-497.
14. Mutscher, H. (1995). Measurement and Assessment of Soil potassium. Basel,, Switzerland. IPI.
Basel, Switzerland, 102 pp.
15. Bremner, J.M., Sparks, D.L., Page, A.L., Helmke, P.A., Loeppert, R.H., Soltanpour, P.N.,
Tabatabai, M.A., Johnston, C.T. and Sumner, M.E. (1996). Nitrogen-total. In Methods of soil
analysis. Part 3-chemical methods. 1085-1121.

16. Bates, L.S., Waldren, R.P. and Teare, I.D. (1973). Rapid determination of free proline for waterstress
studies. J. Plant and soil, 39, 205-207.
17. Heath, R. L. and Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and
stoichiometry of fatty acid peroxidation. Arch. Biochem Biophys, 125, 189-198.
18. Nelson, N. (1944). A photometric adaptation of the Somogyi method for the determination of
glucose. J. biol. Chem, 153, 375-379.
19. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.T. and Smith, F. (1956). Colorimetric method
for determination of sugars and related substances. Anal. chem, 28, 350-356.
20. Bradford, M.M. (1976). A rapid and sensitive method for the quantification of microgram
quantities of protein utilizing the principle of protein-dye binding. Anal. biochem, 72, 248-254.
21. Davis, B.J. (1964). Disc electrophoresis. II. Method and application to human serum proteins. -
Ann. N.Y. Acad. Sci. USA 121:404-427.
22. Van Loon, L.C. (1971). Tobacco polyphenoloxidases: a specific staining method indicating nonidentity
with peroxidases. Phytochemistry, 10, 503-507.
23. Woodbury, W., Spencer, A. K. and Stahmann, M.A. (1971). An improved procedure using
ferricyanide for detecting catalase isozymes. Anal. Biochem. 44, 301-305.
24. Wendel, J. F. and Weeden, N.F. (1989). Visualization and interpretation of plant isozymes, In
Soltis DE, Soltis PS (ed.), Isozymes in plant biology. 5-45.
25. Burt, R. (2009). Soil Survey Investigations Report No. 51, Version 1.0. In Soil survey field and
laboratory methods manual. USDA-NRCS, National Soil Survey Center, Lincoln, NE.
26. Shaposhnik, V.A. (2007). History of the discovery of potassium and sodium (on the 200th
anniversary of the discovery of potassium and sodium). J. Anal. Chem, 62, 1100-1102. yeki
beshan. Faghat 24 bemone.
27. Mohammadian, R., Khoyi, F.R., Rahimian, H., Moghaddam, M., Ghassemi-Golezani, K. and
Sadeghian, S.Y. (2001). The effects of early season drought on stomatal conductance, leaf-air
temperature different and proline accumulation in sugar beet genotypes. J.Agric.Sci. Technol. 3:
28. Zeeman, SC., Thorneycroft, D., Schupp, N., Chapple, A., Weck, M., Dunstan, H., Haldimann, P.,
Bechtold, N., Smith, A.M. and Smith, S.M. (2004). Plastidial α-glucan phosphorylase is not
required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic
stress. Plant Physiol., 135(2), 849-858.
29. Ahmad, P. and Prasad, M.N.V. (2012). Abiotic Stress Responses in Plants: Metabolism,
Productivity and Sustainability, Springer, New York, NY, USA.
30. Martinez, C. A., Loureiro, M. E., Oliva, M.A. and Maestri, M. (2001). Differential responses of
superoxide dismutase in freezing resistant< i> Solanum curtilobum and freezing sensitive< i>
Solanum tuberosum subjected to oxidative and water stress. Plant Sci. 160(3), 505-515.