Comparative responses of two Trigonella species to salinity and drought stresses in vitro

Document Type : Original Research Papers


School of Biology, and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran


Effects of salinity and drought on growth, contents of proline, malondialdehyde (MDA), protein
and activity of antioxidative enzymes were studied in two Trigonella species. Seeds and
explants of T. foenum-graecum and T. aphanoneura were grown on Murashige and Skoog
medium (MS) complemented with iso-osmotic concentrations of NaCl (0, 100, 150 mM) and
mannitol (0, 180, 275 mM). Growth and relative water content (RWC) of seedlings and calli
decreased by increasing of salinity and drought in both species. In contrast to that of calli,
proline and protein contents increased in seedlings of both species under both stresses. The
increase of proline content in seedlings of T. aphanoneura was higher than that of T. foenumgraecum
under NaCl stress. MDA content in seedlings of T. aphanoneura was higher than that
of T. foenum-graecum and increased in both species under salinity. Among antioxidative
enzymes, catalase (CAT) activity increased continuously in seedlings of T. aphanoneura
comparing to that of T. foenum-graecum. Similar increasing trends were obtained regarding
CAT activities in calli of both species under both stresses. Increase in activities of SOD, CAT
and POX was observed in calli of both species under stress. It seems that undifferentiated calli
respond more regularly to both stresses. Finally, higher proline content and lower amount of
MDA could be considered as criteria for higher tolerance of T. foenum-graecum seedlings
against osmotic stresses.


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