Effects of liquid, temporary immersion bioreactor and solid culture systems on micropropagation of Lilium ledebourii via bulblet microscales— An endangered valuable plant with ornamental potential

Document Type: Research Paper


1 Department of Plant Physiology, College of Sciences, University of Tehran, Tehran, Iran

2 Department of Horticultural Sciences, Faculty of Agriculture, University of Tehran, Karaj, Iran


Lilium ledebourii (Baker) Boiss. (Liliaceae) is a critically endangered lily species native to
northern Iran, where it is protected by law. In order to develop a cost effective method for largescale
propagation, the effects of three culture systems (solid, liquid and temporary immersion)
and two types of cytokinins [6-Benzyladenine (BA) and Thidiazuron (TDZ)] were studied on
the in vitro plant regeneration of L. ledebourii. To establish the protocol, we used in vitro
regenerated bulblets obtained from bulb scale segments that were cultured on solid Murashige
and Skoog (MS) media as starting material. The bulblet microscale transverse thin cell layers
were cultured on MS solid medium containing 3% sucrose and different combinations of plant
growth regulators. Choice of both, the culture system and the type of cytokinin, affected the
differentiation of explants. Two types of calli formed on explants: type I callus was
embryogenic, while type II callus was shoot organogenesis. The highest percentage (94%) of
embryogenic callus was obtained when calli were transferred on MS solid media supplemented
with 0.54 μM α-Naphthaleneacetic acid (NAA) and 0.44 μM BA. In addition, it was also
observed that the use of temporary immersion bioreactor resulted in a significantly lower
amount of shoot organogenesis rather than solid culture systems. Seventy percent of the
plantlets were successfully acclimatized to ex–vitro conditions and were phenotypically similar
to the mother plants.


Main Subjects

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