Antibacterial activity of some actinomycetes isolated from soils of Alborz province, Iran

Document Type: Research Paper


1 1. Department of Cellular and Molecular Science, School of Biological Science, Kharazmi University, Karaj, Iran 2. Cell-Development and biodiversity research center, Karaj, Iran

2 Department of Microbiology, School of Biological Science, Ferdowsi University, Mashhad, Iran

3 Department of Cellular and Molecular Science, School of Biological Science, Kharazmi University, Karaj, Iran


Screening of microorganisms for the production of novel antibiotics has been intensively
pursued for many years by scientists. Actinomycetes have the capability to synthesize many
different antibiotics. A total of 69 actinomycete isolates were recovered from soil samples
collected from Alborz Province. Selected colonies (rough, chalky) of actinomycetes were
purified. All screened isolates were identified morphologically and physiologically. And all of
them belong to the Streptomyces genus. These were then assessed for their antibacterial activity
against pathogenic bacteria. Four pathogenic test strains were used in this study including
Staphylococcus aureus ATCC 6538, Bacillus subtilis ATTC 6633, Klebsiella pneumoniae
ATTC 10031, and Escherichia coli ATCC 29998. Determination of antibacterial activities of
isolated actinomycetes performed by using modified spektra-plak method and Mueller Hinton
agar (Oxoid) plates. Antagonism was detected by formation of inhibition zone. Results of the
study indicated that 12 isolates were active against S. aureus, 15 isolates against B. subtilis, six
isolate against K. pneumonia, and four isolate were active against E. coli.


Main Subjects

1. Solano, G., Rojas−Jiménez, K., Jaspars, M. and Tamayo−Castillo, G. (2009) Study of the
diversity of culturable actinomycetes in the North Pacific and Caribbean coasts of Costa Rica.
Antonie van Leeuwenhoek 96: 71–78.
2. Horan, A.C. (1999) Secondary metabolite production actinomycetes other than Streptomyces. In:
Flickinger, M.C. and Drew, S.W. (ed.) Encyclopedia of Bioprocess Technology: Fermentation,
Biocatalysis and Bioseparation, New York: Wiley and Sons. 2333–2348.
3. Lazzarini, A., Caveletti, L., Toppo, G. and Marinelli, F. (2000) Rare genera of actinomycetes as
potential producers of new antibiotics. Antonie van Leeuwenhoek, 78, 399–405.
4. McCarthy, A.J. and Williams, S.T. (1992) Actinomycetes as agents of biodegradation in the
enviroment– a review. Gene, 115, 189–192.
5. Sanglier, J.J., Haag, H., Huck, J.A. and Fehr, T. (1996) Review of actinomycetes compounds
1990–1995. Expert Opinion and Investigational Drugs, 5, 207–223.
6. Oskay, M, Üsame, T.A. and Azeri, C. (2004) Antibacterial activity of some actinomycetes
isolated from farming soils of Turkey, Afr. j.biotechnol., 3, 441-446.
7. Shirling, E.B. and Göttlieb, D. (1966) Methods for characterization of Streptomyces species. Int.
J. Syst. Bacteriol., 16, 313-340.
8. Korn-Wendisch, F. and Kutzner, H.J. The family Streptomycetaceae. The Prokaryotes. A
handbook on the biology of bacteria: ecophysiology,isolation, identification, applications,
Chapter 41.
9. Thakur, D., Yadav, A., Gogoi, B.K. and Bora, T.C. (2007) Isolation and screening of
Streptomyces in soil of protected forest areas from the states of Assam and Tripura, India, for
antimicrobial metabolites. J. Mycol. Med., 17, 242-249.
10. Locci, R. (1989) Streptomycetes and related genera. In: Williams, S.T., Sharpe, M.E., Holt, J.G.,
(ed). Bergey’s manual of systematic bacteriology. Baltimore: Williams and Wilkins, 2451-2493.
11. Madigan, M.T., Martiko, J.M. and Parker, J. (1997) Antibiotics: Isolation and characterization,
in: Brook Biology of Microorganisms, 8th edn. Prentice-Hall International Inc. New Jersey, 440-
12. Anderson, A.S. and Wellington, M.H.E. (2001) The taxonomy of Streptomyces and related
genera. Int. J. of Syst. Evol. Microbiol., 51, 797-814.
13. Wendisch, F.K. and Kutzner, H.J. (1991) The family Streptomycetaceae. In: Ballows, A. (Ed.)
Prokaryotes, Springer Verlag. Second edition. Vol. 1, 922-995.
14. Williams, S.T., Goodfellow, M. and Alderson, G. (1989) Genus Streptomyces Waksman and
Henrici 1943.339AL. In: Bergey' s Manual of Systematic Bacteriology. Williams & Wilkins
Company, Baltimore. Vol: 4, 2452-2492.
15. Lacey, J. (1973) Actinomycetales: Characteristics and Practical ─░mportance. Edited by Sykes, G.
and Skinner, F., The Society for Applied Bacteriology Symposium Series. Academic Press
London-New York No. 2.
16. Ouhdouch, Y., Barakate, M., and Finance, C. (2001) Actinomycetes of Moroccan habitats:
isolation and screening for antifungal activities. Eur. J. Soil. Biol., 37, 69-74.

17. Saadoun, I. and Gharaibeh, R. (2003) The Streptomyces flora of Badia region of Jordan and its
potential as a source of antibiotic-resistant bacteria. J. Arid. Environ., 53, 365-371.
18. Waksman, S.A. (1961) The Actinomycetes, Classification, Identification and Description of
Genera and Species. Baltimore: The Williams and Wilkins Company. Vol. 2, 61-292.
19. Iwai, Y. and Omura, S. (1992) Cultural conditions for screening of new antibiotics. J. Antibiot.,
34, 123-141.
20. Passari, A.K., Mishra, V.K., Saikia, R., GuptaV.K., and Singh. B.P. (2015) Isolation abundance
and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and
screening for their invitro antimicrobial biosynthetic potential. Frontiers in Microbiol., 6, 273
21. Mrinalini, J.S. and Padmavathy, S. (2014) Isolation screening and characterization of endophytic
PGRP actinomycetes present commonly Innerm & Tulsi leaves invitro study (Tomato). Int. J.
Rec. Sci. research, 5 (3), 574-579.
22. Shilpa, A.J., Soni, R., Patel, H., Prajapati B. and Patel, G. (2014) Screening, isolation and
characterization of keratin degrading actinomycetes: Streptomyces sp. and Saccharothrix
xinjiangensi and analyzing their significance for production of keratinolytic protease and feed
grade aminoacids. Int. J. Curr. Microbiol. App. Sci., 3(9), 940-955.
23. Walsh, S.E., Maillard, J.Y., Russell, A.D., Catrenich, C.E., Charbonneau, D.L. and Bartolo, R.G.
(2003) Activity and mechanisms of action of selected biocidal agents on Gram-positive and –
negative bacteria. J. App. Microbiol. 94 (2) 240–247.
24. Goljanian Tabrizi, S., Hamedi, J. and Mohammadipanah, F. (2014) Screening of actinomyectes
against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active
strains. I. J. M., 5 (4) 356-365.
25. Cross, T. (1989) Growth and Examination of Actinomycetes Some Guidelines. In Bergey' s
Manual of Systematic Bacteriol. Williams & Wilkins Company, Baltimore, 4., 2340-2343.
26. Lechevalier, H.A. (1989) The Actinomycetes III, A Practical Guide to Generic Identification of
Actinomycetes. Bergey's Manual of Systematic Bacteriology. Williams & Wilkins Company,
Baltimore. Vol. 4., 2344-2347.
27. Goodfellow, M. (1989) Suprageneric Classification of Actinomycetes. In Bergey's Manual of
Systematic Bacteriology. Williams & Wilkins Company, Baltimore, 4, 2333-2339.