Antibacterial activity of some actinomycetes isolated from soils of Alborz province, Iran

Document Type : Original Research Papers

Authors

1 1. Department of Cellular and Molecular Science, School of Biological Science, Kharazmi University, Karaj, Iran 2. Cell-Development and biodiversity research center, Karaj, Iran

2 Department of Microbiology, School of Biological Science, Ferdowsi University, Mashhad, Iran

3 Department of Cellular and Molecular Science, School of Biological Science, Kharazmi University, Karaj, Iran

Abstract

Screening of microorganisms for the production of novel antibiotics has been intensively
pursued for many years by scientists. Actinomycetes have the capability to synthesize many
different antibiotics. A total of 69 actinomycete isolates were recovered from soil samples
collected from Alborz Province. Selected colonies (rough, chalky) of actinomycetes were
purified. All screened isolates were identified morphologically and physiologically. And all of
them belong to the Streptomyces genus. These were then assessed for their antibacterial activity
against pathogenic bacteria. Four pathogenic test strains were used in this study including
Staphylococcus aureus ATCC 6538, Bacillus subtilis ATTC 6633, Klebsiella pneumoniae
ATTC 10031, and Escherichia coli ATCC 29998. Determination of antibacterial activities of
isolated actinomycetes performed by using modified spektra-plak method and Mueller Hinton
agar (Oxoid) plates. Antagonism was detected by formation of inhibition zone. Results of the
study indicated that 12 isolates were active against S. aureus, 15 isolates against B. subtilis, six
isolate against K. pneumonia, and four isolate were active against E. coli.

Keywords

Main Subjects

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Progress in Biological Sciences
Volume 5, Issue 2 - Serial Number 2
Summer & Autumn
July 2015
Pages 159-167

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