Transcription of growth hormone mRNA as a molecular marker of egg quality in Siberian sturgeon (Acipenser baerii)

Document Type: Research Paper


1 Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmehsara, Iran

2 Iranian Fisheries Science Research Institute/Agriculture Research Education Extension, Organization, Tehran, Iran


The Siberian sturgeon (Acipenser baerii) is an economically important species. Sturgeon stock
assessment during fingerling production is considered to be one of the most difficult phases of
hatchery rearing. The present study investigated the expression of growth hormone (GH)
mRNA in unfertilized eggs, fertilized eggs, eyed eggs (2 day before hatching), non-viable eggs
and newly-hatched Siberian sturgeon. Knowledge about the expression of GH during egg and
embryo development can help determine the quality of the eggs. This can help predict larval
viability and, to some extent, directly determine the growth and survival of young fish. Little is
known about the effect of this hormone on egg quality in sturgeon. The present study analyzed
relative GH mRNA expression using the 2-ΔΔCT method. Ribosomal protein L6 (RPL6)
transcripts were used as the housekeeping gene for normalization of GH mRNA transcription.
The highest levels of GH mRNA were found in eyed eggs and the lowest levels were detected in
non-viable eggs (P< 0.05). These findings suggest GH mRNA as a potential marker for egg
quality in Siberian sturgeon.


Main Subjects

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