A quantitative competitor PCR assay to detect genetically modified roundup-ready soybeans in commercially sold foods in Iran

Document Type: Original Research Papers

Authors

1 1. Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran 2. Department of Biology, Faculty of Science, Lorestan University, Khoramabad, Iran

2 Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

3 Department of Biology, Faculty of Science, Lorestan University, Khoramabad, Iran

Abstract

Due to ever-increasing global diffusion and related socio-economic implications, the detection
of genetically modified organisms (GMOs) is very important. In this study, we design a plasmid
containing two genes in mutated form as construct-specific (cp4 epsps) and event-specific
(pd35S). It is applied for quantitative-competitor (QC) PCR as a simple and reliable method for
the detection of GM food. This plasmid is calibrated with the external standard of the IRMM
(The Institute for Reference Materials and Measurements), and then used to detect the presence
of cp4 epsps and pd35S sequences in five foods derived from GM round-ready (RR) soya sold
commercially in Iran. The results indicate the presence of GM RR soya in these products,
quantitatively. In order to detect whether they contain more or less than 1% RR soya DNA, QCPCR
with various amounts of DNA plasmids as a standard was performed. The results show
that this plasmid can be used as a calibrator for 1% cp4 epsps and pd35S, and that it can also be
applied instead of 1% IRMM genomics.
 

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