Genetic Variation of Choline Dehydrogenase Gene in Idiopathic Male Infertility

Document Type: Original Research Papers


1 Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

2 Department of Biology, Faculty of sciences, University of Guilan, Rasht , Iran Tel:0098-9112446281 Email:

3 Cellular and molecular research center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

4 - Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran


Infertility can be caused by an unexplained reduction in semen quality in males who present as
normal on physical examination and endocrine testing. There is some evidence that aberrant
metabolism of micronutrients such as choline may play a causative role in male factor
infertility. Choline is a crucial factor in the regulation of sperm membrane structure and
motility, and this nutrient plays an important role in the maturing and fertilizing capacity of
spermatozoa. In the present study, we explored the contribution of the choline dehydrogenase
gene polymorphism located in the codon 78 (CHDH +432G>T), one of the basic enzymes of
choline metabolism, to idiopathic male infertility. In this study, 50 infertile men and 50 fertile
men of the Guilan population were selected. Genomic DNA was extracted from peripheral
blood. Genotypes were determined by polymerase chain reaction (PCR) and restriction
fragment length polymorphism (RFLP). Statistical analysis was performed using MedCalc
software (v12.1.4.0). A significant difference was observed between patients and healthy
subjects in the distribution of G and T alleles. The prevalence of genotype frequencies of
CHDH +432 GG, GT, and TT were 28%, 50%, and 22%, respectively, in patients, while in
healthy subjects they were 52%, 36%, and 12%, respectively. In other words, there was a
significant difference in the genotype distribution of CHDH +432G>T in patients compared
with controls (Ρ<0.05). This finding suggests a possible influence of this gene polymorphism on
idiopathic male infertility.


Main Subjects