Biological Removal of Dibenzothiophene from Soil and Changes to soil Sulfate by White-Rot Fungus Phanerochaete chrysosporium

Document Type: Original Research Papers

Authors

1 Biotech Lab, Department of soil science, university of Tehran, Iran

2 Biotech Lab, Department of Soil Science, University College of Agriculture and Natural Resources, University of Tehran, Tehran, Iran

Abstract

This study investigated biodegradation of dibenzothiophene (DBT) in marsh soil spiked by
white-rot fungus Phanerochaete chrysosporium. Soil samples were spiked with 100 ppm DBT
and incubated at 30°C in a dark chamber for 30 days. Samples were evaluated for pH, Mnperoxidase
activity, sulfate ion concentration and growth during the tests. Results showed
maximum levels of pH, Mn-peroxidase and sulfate concentrations at 8.19, 66 U L-1 and 31.21
mg kg-1 respectively. Colony forming unit assay determinations for contaminated soil samples
showed that the fungus was able to grow and use dibenzothiophene as a source of carbon and
energy. GC analysis of contaminated samples compared to control samples, demonstrated
degradation of DBT by Phanerochaete chrysosporium. There was no significantly improved
effect on degradation when the treatment was performed in presence of soil micro flora. The
system attained a high rate of DBT degradation at approximately 83.34%, the 30 day test
operation under optimal conditions. After 20 days, the sulfate ion concentration in soil samples
had almost doubled.

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