Document Type: Original Research Papers
Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran
The anionic surfactant sodium dodecyl sulfate (SDS) was degraded by novel strain of
Pseudomonas aeruginosa KGS under accession No. JQ328193, which was isolated from car
wash wastewater. The purpose of this research was to study different optimization conditions
required for enhancing the biodegradation of sodium dodecyl sulfate P. aeruginosa KGS.
Influence of different Physicochemical factors such as nitrogen and carbon sources, pH,
temperature, inoculation percent and different concentrations of SDS on the biodegradation of
SDS were investigated by measuring the degradation rate of SDS using methylene blue active
substance (MBAS) method. The optimum conditions determined for the this selected bacterium
strain for degradation of SDS were 1.5mM SDS, inoculation percent 7%, pH 7.5, temperature
37°C, ammonium nitrate (nitrogen source) when basal salt medium was supplemented with
glucose as a co substrate. This bacterium is able to degrade about 98% of the SDS after 24h of
incubation under optimized conditions of biodegradation. The results presented in this research
indicate that Pseudomonas aeruginosa is a suitable candidate for SDS biodegradation.