Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased the organogenesis response and produced 4.4 bulblets per explants. Two months later, the bulblets were transferred to MS PGRs-free medium. Bulblets were successfully transplanted to the soil after a cold treatment of 8 weeks, with a survival rate of 85%.
Mirmasoumi,M , Azadi,P , Sharafi,A , Ntui Otang,V and Mii,M . (2013). Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer. Progress in Biological Sciences, 3(2), 117-122. doi: 10.22059/pbs.2013.35828
MLA
Mirmasoumi,M , , Azadi,P , , Sharafi,A , , Ntui Otang,V , and Mii,M . "Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer", Progress in Biological Sciences, 3, 2, 2013, 117-122. doi: 10.22059/pbs.2013.35828
HARVARD
Mirmasoumi M, Azadi P, Sharafi A, Ntui Otang V, Mii M. (2013). 'Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer', Progress in Biological Sciences, 3(2), pp. 117-122. doi: 10.22059/pbs.2013.35828
CHICAGO
M Mirmasoumi, P Azadi, A Sharafi, V Ntui Otang and M Mii, "Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer," Progress in Biological Sciences, 3 2 (2013): 117-122, doi: 10.22059/pbs.2013.35828
VANCOUVER
Mirmasoumi M, Azadi P, Sharafi A, Ntui Otang V, Mii M. Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer. Progress in Biological Sciences. 2013;3(2):117-122. doi: 10.22059/pbs.2013.35828
