Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer

Document Type : Original Research Papers


1 Department of Botany, School of Biology, Collage of Science, University of Tehran, Tehran, Iran

2 Tissue Culture and Genetic Engineering Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran

3 Novin Giti Gene (NGG) Co., Bio-Incubator Center NIGEB, Tehran, Iran

4 Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City, Chiba 271-8510, Japan


Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased the organogenesis response and produced 4.4 bulblets per explants. Two months later, the bulblets were transferred to MS PGRs-free medium. Bulblets were successfully transplanted to the soil after a cold treatment of 8 weeks,
with a survival rate of 85%.