Xanthomonas citri subsp citri (Xcc) is responsible for one of the most destructive diseases of citrus fruits, Asiatic citrus canker. Based on the bacterial host range, the pathogenic forms, A, A*, and Aw have been identified. A*-type strains are not well characterized and they have been reported to differ from other A-type strains on the basis of biochemical and genetic characteristics. In this study, we attempted to develop a medium to mimic in planta conditions for inducing protein secretion of the A*-type strain-NIGEB-088. We also attempted to achieve efficient extraction of Xcc A*-type extracellular proteins, the key mediators of plant-bacterium interaction. Results obtained by this study demonstrate that the m3 (mMM1) can induce Xcc A*-type strains to secret extracellular proteins in amounts detectable by two dimensional gel electrophoresis
(2-DE). We also show that Pyrogallol red-molybdate methanol (PRMM) is more effective for precipitation of Xcc A*-type secreted proteins than other assessed procedures of protein precipitation, i. e. acetone, TCA, and sodium deoxycholate
Mozdoori, N. , Parchami, N. , Kazemipour, N. and Alavi, S. M. (2012). Sample Preparation for Secretome Analysis in A*-Type
Strains of Xanthomonas citri subsp. citri, the Causal Agent of Asiatic Citrus Canker Disease. Progress in Biological Sciences, 2(1), 87-94. doi: 10.22059/pbs.2012.24828
MLA
Mozdoori, N. , , Parchami, N. , , Kazemipour, N. , and Alavi, S. M. . "Sample Preparation for Secretome Analysis in A*-Type
Strains of Xanthomonas citri subsp. citri, the Causal Agent of Asiatic Citrus Canker Disease", Progress in Biological Sciences, 2, 1, 2012, 87-94. doi: 10.22059/pbs.2012.24828
HARVARD
Mozdoori, N., Parchami, N., Kazemipour, N., Alavi, S. M. (2012). 'Sample Preparation for Secretome Analysis in A*-Type
Strains of Xanthomonas citri subsp. citri, the Causal Agent of Asiatic Citrus Canker Disease', Progress in Biological Sciences, 2(1), pp. 87-94. doi: 10.22059/pbs.2012.24828
CHICAGO
N. Mozdoori , N. Parchami , N. Kazemipour and S. M. Alavi, "Sample Preparation for Secretome Analysis in A*-Type
Strains of Xanthomonas citri subsp. citri, the Causal Agent of Asiatic Citrus Canker Disease," Progress in Biological Sciences, 2 1 (2012): 87-94, doi: 10.22059/pbs.2012.24828
VANCOUVER
Mozdoori, N., Parchami, N., Kazemipour, N., Alavi, S. M. Sample Preparation for Secretome Analysis in A*-Type
Strains of Xanthomonas citri subsp. citri, the Causal Agent of Asiatic Citrus Canker Disease. Progress in Biological Sciences, 2012; 2(1): 87-94. doi: 10.22059/pbs.2012.24828
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