Assessment of salt tolerance in transgenic tobacco (Nicotiana tobacum L.) plants expressing the AUX gene


University of Isfahan-P.H.D


Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production
as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes
carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development
of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and
plants regenerated from transgenic roots were subjected to 300 mM NaCl. Transgenic plants
showed higher levels of salt tolerance compared to non-transgenic plants. This could be due to
over expression of the AUX gene in transformed hairy roots and plants regenerated from transgenic
roots. © 2011 Progress in Biological Sciences, Vol. 1, No.1, 17-23.