Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay

Masoudipour, Elham; Mousavi, Seyed Latif; Basiri, Mohsen

doi:10.22059/pbs.2011.22454

Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay

Authors

Elham Masoudipour ¹

Seyed Latif Mousavi ²

Mohsen Basiri ³

¹ Khatam University-P.H.D

² Shahed University-P.H.D

³ Tarbiat Modares University

10.22059/pbs.2011.22454

Abstract

Development of potent new anti-Shigella agents for rapid and specific detection and treatment is
of great importance. Aptamers, nucleic acid oligomers capable of specific binding to a wide range
of non-nucleic acid targets, may be of value for this purpose. In the present study, we used a Systematic
Evolution of Ligands by Exponential enrichment (SELEX) process to select DNA aptamers
that bind to whole S. sonnei cells. The resulting aptamers exhibited specificity in binding only
to S. sonnei cells. Five unique DNA sequences were isolated from the aptamer cocktail by cloning,
among which ASA4 showed the highest affinity. © 2011 Progress in Biological Sciences, Vol. 1,
No.1, 11-15.

Keywords

DNA aptamer

ELASA

Exponential

SELEX

Shigella sonnei