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Progress in Biological Sciences
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Summer and Autumn 2011, Page 1-55
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Winter and Spring 2011, Page 1-114
Masoudipour, E., Mousavi, S., Basiri, M. (2011). Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay. Progress in Biological Sciences, 1(1), 11-25. doi: 10.22059/pbs.2011.22454
Elham Masoudipour; Seyed Latif Mousavi; Mohsen Basiri. "Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay". Progress in Biological Sciences, 1, 1, 2011, 11-25. doi: 10.22059/pbs.2011.22454
Masoudipour, E., Mousavi, S., Basiri, M. (2011). 'Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay', Progress in Biological Sciences, 1(1), pp. 11-25. doi: 10.22059/pbs.2011.22454
Masoudipour, E., Mousavi, S., Basiri, M. Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay. Progress in Biological Sciences, 2011; 1(1): 11-25. doi: 10.22059/pbs.2011.22454

Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay

Article 2, Volume 1, Issue 1, Winter and Spring 2011, Page 11-25  XML PDF (67 K)
DOI: 10.22059/pbs.2011.22454
Authors
Elham Masoudipour1; Seyed Latif Mousavi2; Mohsen Basiri3
1Khatam University-P.H.D
2Shahed University-P.H.D
3Tarbiat Modares University
Abstract
Development of potent new anti-Shigella agents for rapid and specific detection and treatment is
of great importance. Aptamers, nucleic acid oligomers capable of specific binding to a wide range
of non-nucleic acid targets, may be of value for this purpose. In the present study, we used a Systematic
Evolution of Ligands by Exponential enrichment (SELEX) process to select DNA aptamers
that bind to whole S. sonnei cells. The resulting aptamers exhibited specificity in binding only
to S. sonnei cells. Five unique DNA sequences were isolated from the aptamer cocktail by cloning,
among which ASA4 showed the highest affinity. © 2011 Progress in Biological Sciences, Vol. 1,
No.1, 11-15.
Keywords
DNA aptamer; ELASA; Exponential; SELEX; Shigella sonnei
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