University of Tehran PressProgress in Biological Sciences1016-10581220111001Evidence for an association between Wnt-independent
-catenin intracellular localization and ovarian apoptotic events in normal and PCO-induced rat ovaryEvidence for an association between Wnt-independent
-catenin intracellular localization and ovarian apoptotic events in normal and PCO-induced rat ovary1102428410.22059/pbs.2011.24284ENHosseinGhamartajUniversity of Tehran,Department of Animal BiologyManijehKhanmohammadiUniversity of Tehran,Department of Animal BiologyNedaJarooghiUniversity of Tehran,Department of Animal BiologySomayeKazemnejadAvicenna Research Institute, Tehran, Department of Stem Cell & EmbryologyJournal Article19700101The association of secreted frizzled related protein type 4 (Sfrp4) as an antagonist of Wnt mole-cules in apoptotic events has been reported previously. Moreover, its increased expression has been reported in the ovary of women with polycystic ovary (PCO). We have demonstrated in-creased Sfrp4 in PCO-induced rat ovary related to an increased number of apoptotic follicles showing nuclear ?catenin subcellular localization. The aims of present study were twofold 1) to ascertain nuclear ?catenin presence with apoptosis by using immunolocalization of Bax and active cleaved casapase-3, and 2) to elucidate whether Sfrp4 could be an inducer of apoptosis by using isolated rat granulosa cell culture in the presence of recombinant human SFRP4. To this end, immuno-expression of two key molecules in Wnt signaling, GSK3? and ?-catenin and apoptotic markers were investigated in normal and PCO-induced rat ovary by daily administration of testos-terone propionate (TP) for four weeks. We showed that in PCO-induced as well as in normal ova-ries there was nuclear or cytoplasmic subcellular localization of GSK3? and a weak pGSK3?ser9 immuno-staining in apoptotic granulosa cells. Interestingly, intracellular ?catenin localization was observed in Bax and active caspase-3 positive granulosa cells in normal as well as in PCO-induced rat ovary. Treatment of granulosa cells with rhSFRP4 showed co-localization of nuclear subcellu-lar ?-catenin and active caspase-3 as revealed by double immuno-fluorescence. Our results suggest that rhSFRP4 induces apoptosis and that there is an association between Wnt-independent ?-catenin nuclear subcellular localization and apoptotic events of rat ovary.https://pbiosci.ut.ac.ir/article_24284_0a09e7842d23011283d6999caddc6f79.pdfUniversity of Tehran PressProgress in Biological Sciences1016-10581220111001Predation in two species of leech under laboratory
conditionsPredation in two species of leech under laboratory
conditions11152428510.22059/pbs.2011.24285ENKavehDarabi DarestaniPHD.University of TehranMasoumehMalekPHD.University of TehranJournal Article19700101Two 60 day experiments on predation behavior of leeches were conducted concurrently. In the first, medicinal leeches, Hirudo orientalis (Utevsky and Trontelj, 2005) were fed to satiation on bovine blood and subsequently transferred into two large opaque plastic containers of equal size but with differing temperatures, 6-7?C and 23-24?C, containing starved Erpobdella octoculata (L.). At the higher temperature E. octoculata rapidly attacked and swallowed juvenile and adult medicinal leeches. This also occurred at the lower temperature but took place more slowly. In the second experiment, under similar physical conditions, starved medicinal leeches and satiated E. octoculata were studied. Starved H. orientalis attached to and sucked ingested blood from juvenile E. octoculata but did not consume them. H. orientalis did not feed on adult E. octoculata. These observations confirm interspecific predation between two families of leeches, Hirudinidae and Erpobdellidae. Numbers and wet mass of prey leeches decreased rapidly at the higher temperature, especially when E. octoculata was the predator. ? 2011 Progress in Biological Sciences. Vol. 1, No. 2, 11-15.https://pbiosci.ut.ac.ir/article_24285_55431db0f7fbb1526bffec8d7a0c0c04.pdfUniversity of Tehran PressProgress in Biological Sciences1016-10581220111001History of study and checklist of the scorpion fauna (Arachnida: Scorpiones) of IranHistory of study and checklist of the scorpion fauna (Arachnida: Scorpiones) of Iran16232428610.22059/pbs.2011.24286ENOmidMirshamsiPHD.Ferdowsi University of MashhadAlirezaSariPHD.University of TehranShidokhtHosseiniePHD.Shiraz UniversityJournal Article19700101An updated checklist of the scorpion fauna of Iran, as well as a brief history of taxonomic research on scorpions of Iran is presented. The checklist is based on records of scorpion species whose presence has been confirmed in Iran through field expeditions, examination of scorpion collections, literature review, and personal communications from researchers. The scorpion fauna consists of 51 species (30 endemic to Iran) belonging to 18 genera and four families. ? 2011 Progress in Biological Sciences. Vol. 1, No. 2, 16-28. An updated checklist of the scorpion fauna of Iran, as well as a brief history of taxonomic research on scorpions of Iran is presented. The checklist is based on records of scorpion species whose presence has been confirmed in Iran through field expeditions, examination of scorpion collections, literature review, and personal communications from researchers. The scorpion fauna consists of 51 species (30 endemic to Iran) belonging to 18 genera and four families. ? 2011 Progress in Biological Sciences. Vol. 1, No. 2, 16-28.https://pbiosci.ut.ac.ir/article_24286_a9ae05ef6695b84add07582d03f9f7d1.pdfUniversity of Tehran PressProgress in Biological Sciences1016-10581220111001Reconstruction of early Neolithic/Bronze Age population diversity in the Shamanka II cemetery at Lake Baikal
using mtDNA polymorphismReconstruction of early Neolithic/Bronze Age population diversity in the Shamanka II cemetery at Lake Baikal
using mtDNA polymorphism29352428710.22059/pbs.2011.24287ENHamedVahdati NasabPHD.TarbiatModares UniversityJournal Article19700101Mitochondrial deoxyribonucleic acid (mtDNA) polymorphisms were examinedin bone samples of individuals buried inan early Neolithic (c. 5800–4900 BCE) hunter-gatherer cemetery, Shamanka II, located atthe southwestern tip of Lake Baikal, Siberia. The mainobjective was to compare the mtDNA polymorphisms observed at Shamanka II to those previously reportedfrom the Lokomotiv (early Neolithic) and Ust'-Ida (Bronze Age) cemeteries in the same general region also known asthe Cis-Baikal. All three cemetery populations comprised remains containingmtDNAhaplogroupscharacterized as being of Asian origin (haplogroups A, C, D, F, G2a, and U5a). The results confirmed that haplogroup frequency distributions amongNeolithic populations in southern Siberiawere similar, and differed significantly fromhaplogroup frequency distributionsamongthe Bronze Agepopulations.? 2011 Progress in Biological Sciences. Vol. 1, No. 2, 29-35.https://pbiosci.ut.ac.ir/article_24287_77ea873d33bd533355846d667a263c3b.pdfUniversity of Tehran PressProgress in Biological Sciences1016-10581220111001Palynological study of Nonea (Boraginaceae-Boragineae) in IranPalynological study of Nonea (Boraginaceae-Boragineae) in Iran36432428810.22059/pbs.2011.24288ENAtiehNejhad Falatoury, PHD.Alzahra UniversityManijehPakravanPHD, Alzahra UniversityFaribaSharifiniaPHD.Islamic Azad University- Tehran North BranchJournal Article19700101Nonea Medik. is a genus of the Boraginaceae tribe Boragineae, with approximately 35 species worldwide. Examination of their palynological characters supported the separation of taxa, which is usually based on morphological and micromorphological reproductive features. Pollen grains of 10 taxa representing three groups of taxa in Iran, the N. caspica group, the N. persica group and the N. lutea group were studied, but N. rosea and N. turcomanica were not included. The results differed from previous studies in Iran. The N. caspica group, including N. caspica, N. melanocar-pa, and N. diffusa has 5-6-zonocolporate pollen grains with a psilate-punctate tectum. The N. per-sica group, including, N. p. var. persica, var. suchtelenioides, and N. pulla have 4-zonocolporate pollen grains with a psilate-punctate tectum. Finally the N. lutea group, including N. lutea var. lu-tea and N. l. var. flavescens, have 5-zonocolporate pollen grains with a reticulate-micro reticulate tectum. N. rosea and N. turcomanica have 5-zonocolporate pollen grains with a scrobiculate tec-tum ? 2011 Progress in Biological Sciences. Vol. 1, No. 2, 36-43.https://pbiosci.ut.ac.ir/article_24288_138627b71044d6916dba0f8ee506ccb9.pdfUniversity of Tehran PressProgress in Biological Sciences1016-10581220111001Preliminary data suggest possible association between
IL-32 expression level and time of MS attackPreliminary data suggest possible association between
IL-32 expression level and time of MS attack44492428910.22059/pbs.2011.24289ENMehrzadRoghaniPHD.University of TehranFereidounMahboudiPHD.Pasteur Institute of IranMohammad AliSahraianPHD.Tehran University of Medical SciencesMasoudEtemadifarPHD.Isfahan University of Medical SciencesElaheElahiPHD.of Mathematics, Statistics and Computer Science, College of Science, University of Tehran, Tehran, IranJournal Article19700101IL-32 and TNF? are important cytokines in autoimmune and inflammatory diseases. IL-32 has not been previously studied with respect to MS. Here, we report IL-32 and TNF? transcript levels in peripheral blood mononuclear cells of MS patients and control individuals by real time PCR. A significant difference in IL-32 and TNF? mRNA levels of patients as compared to controls was not observed. However, patients with more recent attacks had highly significant increased levels of IL-32 mRNAs. As the sample size was small, this potentially relevant preliminary observation needs to be corroborated using a larger number of patients with well defined clinical features..? 2011 Progress in Biological Sciences. Vol. 1, No. 2, 44-49.https://pbiosci.ut.ac.ir/article_24289_688b1a8ec37c08d510821b230c288ff0.pdfUniversity of Tehran PressProgress in Biological Sciences1016-10581220111001New Records of Nostocalean Cyanobacteria from Rice Fields in the Golestan Province in North-East of IranNew Records of Nostocalean Cyanobacteria from Rice Fields in the Golestan Province in North-East of Iran50552429010.22059/pbs.2011.24290ENRoghayehSiahbalaeiPHD.University of IsfahanSaeedAfsharzadehPHD.University of IsfahanShadmanShokraviPHdD., Islamic Azad University.-Branch Gorgan, GorganJournal Article19700101The algal flora of five stations from paddy-fields of Golestan Province was investigated between October 2006 and September 2007. Eight filamentous heterocystous Nostocacean are recorded here for the first time from paddy fields of iran: Cylindrospermum licheniforme, Cylindrospermum stangnale, Cylindrospermopsis raciborskii, Calothrix ghosei, Gloeotrichia longiarticulata, Aulosira implexa var. crassa, Tolypothrix conglutionata var colorata, Scytonema multiramosum. Cylindrospermum stagnale, Aulosira implexa, Scytonema multiramosum, Tolypothrix conglutionata were dominant species in all stations and seasons, but Gloeotrichia longiarticulata and Cylindrospermopsis raciborskii were observed mainly during the spring and summer. Morphological characteristics of these species are described in detail and information about their ecological distribution is given.? 2011 Progress in Biological Sciences. Vol. 1, No. 2, 50-55.https://pbiosci.ut.ac.ir/article_24290_5222e0d86e57509a44632e499ce2f314.pdf