University of Tehran Press Progress in Biological Sciences 1016-1058 6 2 2017 09 18 Using petrochemical wastewater for synthesis of cruxrhodopsin as an energy capturing nanoparticle by Haloarcula sp. IRU1 151 157 63026 10.22059/PBS.2016.590017 EN Mojtaba Taran Arina Monazah Mehran Alavi Journal Article 2016 04 20 In this study, the feasibility of cruxrhodopsin (CR) production as a multifunctional nanoparticle was investigated and optimized by <em>Halorcula</em>sp. IRU1, a novel halophile Archaea isolated from Urmia Lake, Iran in batch experiments. In this case, Taguchi method was used for effect measurement of three important factors (petrochemical wastewater, yeast extract and KH<sub>2</sub>PO<sub>4</sub>) on CR production. Results illustrated that the petrochemical wastewater concentration was the meaningful factor in CR synthesis. The optimum factor levels for petrochemical wastewater concentration, yeast extract and KH<sub>2</sub>PO<sub>4 </sub>were 2% (w/v), 0.2% (w/v) and 0.004% (w/v), respectively. Also, under these conditions, the predicted value for CR production was about 44.24%. Therefore, this investigation demonstrated that <em>Haloarcula </em>sp. IRU1 has a high potential for synthesis of CR from petrochemical wastewater. Urmia Lake Multifunctional nanoparticles Taguchi Method Biotechnology bioremediation https://pbiosci.ut.ac.ir/article_63026_f15329ce4995e050431c510e0706f71d.pdf