TY - JOUR ID - 53957 TI - Isolation and identification of new beneficial bacterial strains from rhizosphere of Citrus sinensis orchards JO - Progress in Biological Sciences JA - PBS LA - en SN - 1016-1058 AU - Ramezani, Mahnaz AU - Riahi Madvar, Ali AU - Khaleghi, Moj AU - Hemmati, Roohullah AD - Department of Biology, Science and Research branch, Islamic Azad University, Kerman, Iran AD - Department of Biotechnology, Institute of Science and High technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran AD - Department of Biology, Faculty of Sciences, Shahid Bahonar University, Kerman, Iran AD - Department of Biochemistry, faculty of biological sciences,Tarbiat Modares University, Tehran, Iran Y1 - 2015 PY - 2015 VL - 5 IS - 1 SP - 75 EP - 84 KW - Citrus sinensis KW - phosphate solublization KW - Rhizobacter KW - rhizosphere KW - 16S rRNA DO - 10.22059/pbs.2015.53957 N2 - The rhizosphere is the area around the root of a plant occupied by a unique population of usefulbacteria known as plant growth promoting rhizobacteria (PGPR). In this study, the isolation andidentification of rhizobacteria from orange (Citrus sinensis) orchards using 16S rRNA gene, as well asbiological and biochemical assays is reported. Analysis of 16S rRNA gene was confirmed bybiological and biochemical assays and showed that the isolated bacteria belong to the genera Bacilli,Enterobacter and Pseudomonas. Accordingly, Enterobacterkobei MR-R2, Pseudomonas putida MRR5,Bacillus cereus MR-R, Bacillus thuringiensis MR-R1 and Bacillus mycoides MR-R4 wereidentified and registered in GenBank. The results of phosphate solubilization testing revealed that themaximum rate of phosphate solubilization was observed for B. thuringiensis, and E. kobei; however,B. cereus, B. mycoides, and P. putida indicated the least activity. It can be concluded that the isolatedstrains (especially B. thuringiensis) are newly identified phosphate solubilizing bacterial strains,among which B. thuringiensis can be used for growth promotion of orange trees. UR - https://pbiosci.ut.ac.ir/article_53957.html L1 - https://pbiosci.ut.ac.ir/article_53957_27d36d1d5c1216582387ce215a9773ee.pdf ER -