TY - JOUR ID - 32095 TI - Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.) JO - Progress in Biological Sciences JA - PBS LA - en SN - 1016-1058 AU - Karimi Farsad, Laleh AU - Mardi, Mohsen AU - Ebrahimi, Mohammad Ali AD - Genomics Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran AD - Department of Biotechnology, Payame Noor University, Tehran, Iran. Y1 - 2013 PY - 2013 VL - 3 IS - 1 SP - 72 EP - 78 KW - Septoria tritici KW - Wheat KW - Real-time PCR analysis KW - Resistance gene DO - 10.22059/pbs.2013.32095 N2 - Septoria tritici blotch (STB), caused by the ascomycete fungus Mycosphaerella graminicola (asexual stage: Septoria tritici), is one of the most important foliar diseases of wheat. In this research, quantitative expression analysis of five candidate genes for the induction of resistance to STB (PR-1, Bsi, Msr, Per, and Ppi) was conducted in the wheat cultivars ‘Seri 82’ (susceptible) and ‘Frontana’ (resistant). The study used a randomized complete block design with three replications and five time-points (0, 3, 6, 12, and 24hours after inoculation). Evaluation of the expression of five candidate genes by real-time PCR showed that expression of Per, pr-1, Bsi, Msr genes was higher than 5, 3, 3 and 2 fold in treatment vs. control samples in ‘Frontana’ at 12 hours after inoculation respectively. The results indicated the effectiveness of these genes in the conferral of resistance to STB in wheat. UR - https://pbiosci.ut.ac.ir/article_32095.html L1 - https://pbiosci.ut.ac.ir/article_32095_af8b1963a0d79c27a674900c3b547415.pdf ER -