University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
Effect of AtNRT2.1 transgene on HATS nitrate uptake in transgenic Nicotiana plumbaginifolia
1
9
EN
Parzhak
Zoufan
University of Isfahan-P.H.D
mansour_shariati3@yahoo.com
Mansour
Shariati
Shahid Chamran University of Ahvaz-P.H.D
mansour_shariati2@yahoo.com
10.22059/pbs.2011.22453
To investigate the impact of overexpression of AtNRT2.1 transgene from Arabidopsis on nitrate
uptake rate and to understand the regulation of endogenous HATS by nitrate and glutamine amino
acid (Gln) in tobacco plants, wild-type and transgenic (F line) plants grown on soil for 4 weeks
were transferred to hydroponic culture in a controlled-environment with a 16/8h L:D photoperiod
at 24? C/20? C, 70% relative humidity and 150 ? mol. m-2. s-1 light intensity. Nitrate uptake over
time was studied by the ion depletion method. Nitrate uptake in the 2 h exposure to 150 ?M nitrate
showed an increase in the transgenic line compared to the wild-type plant. Subsequently the uptake
trend followed a similar pattern in wild-type plant and transgenic plants
Glutamine,iHATS,Nitrate,nitrate transporter,Tobacco
https://pbiosci.ut.ac.ir/article_22453.html
https://pbiosci.ut.ac.ir/article_22453_accde23a433f5ca7208f63964b231f32.pdf
University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay
11
25
EN
Elham
Masoudipour
Khatam University-P.H.D
slmousavi4@shahed.ac.ir
Seyed Latif
Mousavi
Shahed University-P.H.D
slmousavi3@shahed.ac.ir
Mohsen
Basiri
Tarbiat Modares University
slmousavi2@shahed.ac.ir
10.22059/pbs.2011.22454
Development of potent new anti-Shigella agents for rapid and specific detection and treatment is
of great importance. Aptamers, nucleic acid oligomers capable of specific binding to a wide range
of non-nucleic acid targets, may be of value for this purpose. In the present study, we used a Systematic
Evolution of Ligands by Exponential enrichment (SELEX) process to select DNA aptamers
that bind to whole S. sonnei cells. The resulting aptamers exhibited specificity in binding only
to S. sonnei cells. Five unique DNA sequences were isolated from the aptamer cocktail by cloning,
among which ASA4 showed the highest affinity. © 2011 Progress in Biological Sciences, Vol. 1,
No.1, 11-15.
DNA aptamer,ELASA,Exponential,SELEX,Shigella sonnei
https://pbiosci.ut.ac.ir/article_22454.html
https://pbiosci.ut.ac.ir/article_22454_30dfb48dc35a77aadd7e717615f45d74.pdf
University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
Assessment of salt tolerance in transgenic tobacco (Nicotiana tobacum L.) plants expressing the AUX gene
17
39
EN
Zahra
Zamanzedeh
University of Isfahan-P.H.D
ehsanpou4@yahoo.com
Ali Akbar
Ehsanpour
University of Isfahan-P.H.D
ehsanpou3@yahoo.com
10.22059/pbs.2011.22455
Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production
as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes
carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development
of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and
plants regenerated from transgenic roots were subjected to 300 mM NaCl. Transgenic plants
showed higher levels of salt tolerance compared to non-transgenic plants. This could be due to
over expression of the AUX gene in transformed hairy roots and plants regenerated from transgenic
roots. © 2011 Progress in Biological Sciences, Vol. 1, No.1, 17-23.
Auxin,Salt tolerance,Tobacco,transgenic,transgenic tobacco plant
https://pbiosci.ut.ac.ir/article_22455.html
https://pbiosci.ut.ac.ir/article_22455_6237767ee1319a36d3badbe182bdbe96.pdf
University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
Determination of age and growth of the mudskipper
Periophthalmus waltoni Koumans, 1955 (Actinopterygii:
Perciformes) on the mudflats of Qeshm Island and
Bandar-Abbas, Iran
25
54
EN
Jaleh
Sarafraz
Shahid Beheshti University-P.H.D
asabdoli4@yahoo.com
Asghar
Abdoli
Shahid Beheshti University-P.H.D
asabdoli3@yahoo.com
Bahram
Hassanzadeh
Shahid Beheshti University-P.H.D
asabdoli6@yahoo.com
Ehsan
Kamrani
University of Hormozgan-P.H.D
asabdoli5@yahoo.com
Mohamad Ali
Akbarian
Shahid Beheshti University-p.H.D
asabdoli2@yahoo.com
10.22059/pbs.2011.22456
In order to determine the age structure, growth, and length-weight relationship of the mudskipper,
Periophthalmus waltoni, from coastal mudflats of Qeshm Island, Soroo and Bandar-pol, in Hormuzgan
Province, Iran, we collected 192 individuals in February 2007. Age was determined using
the second pectoral radial bone and year class was based on length-frequency distributions.
Growth was described by the von Bertalanffy growth model. For Bandar-Pol mudskippers, =
141.51 mm, k = 0.46 year-1, and t0 = 0.79 year. For Qeshm mudskippers, = 166.79 mm, k = 0.42
year-1, and t0 = 0.62 year. Overall, males grew larger than females ( = 208 mm vs. 147 mm),
while females grew faster (k = 0.41 vs. 0.19 year-1). The age when the theoretical length was 0 (t0)
was 1.46 year for males and 0.88 for females. Maximum age was estimated to be 4+ years.
age,Growth,Length-weight relationship,Periophthalmus waltoni,Qeshm Island
and Bandar-Abbas
https://pbiosci.ut.ac.ir/article_22456.html
https://pbiosci.ut.ac.ir/article_22456_e5e1d0e6719477376d36a76797fd5e76.pdf
University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
Bioremediation potential of a phenol degrading bacterium, Rhodococcus erythropolis SKO-1
31
70
EN
Mohammad Reza
Soudi
Alzahra University-p.H.D
msoudi3@alzahra.ac.ir
Narjes
Kolahchi
Alzahra University-P.H.D
msoudi2@alzahra.ac.ir
10.22059/pbs.2011.22457
Biodegradation of phenol is a major focus of toxic organic compound degradation by microorganisms
isolated from polluted areas. An increasing number of bacteria and fungi possessing unique
biodegradation capabilities have been isolated in recent years. In this study a new isolate, Rhodococcus
erythropolis SKO-1, from polluted soils in the Tehran oil refinery region, is reported. Identification
was performed by sequencing of 16S rDNA and confirmed by morphological and biochemical
characterization. Phenol was consumed as the sole carbon source, and the ortho pathway
was confirmed as the metabolic route of phenol biodegradation by the isolate. After adaptation,
phenol degradation was approximately 99.64% of an initial concentration of 1000 mg phenol l-1 in
56 h. Enrichment of minimal salt medium by addition of yeast extract resulted in further phenol tolerance
and increased phenol degradation capacity up to 1200 mg phenol l-1.
biodegradation,contaminated soil,monoaromatic hydrocarbons,Oil refinery,Phenol,Rhodococcus erythropolis.
https://pbiosci.ut.ac.ir/article_22457.html
https://pbiosci.ut.ac.ir/article_22457_622163f4adc36a42cbf396a4966ddd30.pdf
University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
Phylogenetic relationships in Ranunculus species
(Ranunculaceae) based on nrDNA ITS and cpDNA trnL-F
sequences
41
87
EN
Sare
Rastipishe
Alzahra University-P.H.D
rastipishe_sare3@yahoo.com
Maneezhe
Pakravan
Alzahra University-P.H.D
rastipishe_sare2@yahoo.com
Akhtar
Tavassoli
Alzahra University-P.H.D
rastipishe_sare@yahoo.com
10.22059/pbs.2011.22458
The genus Ranunculus L., with a worldwide distribution, is the largest member of the Ranunculaceae.
Here, nuclear ribosomal internal transcribed spacer (ITS) sequence data and chloroplast trnLF
sequence data were used to analyze phylogenetic relationships among members of the annual
and perennial (Group Praemorsa, Group Rhizomatosa, Group Grumosa and Group non-Grumosa)
species of Ranunculus in Iran. In the strict consensus tree of nrDNA ITS sequence analyses, seven
sub-clades were described, based on morphological, karyological, palynological, and ecological
features. Within each clade, there were species belonging to more than one Group, and species of a
single Group may fall into different clades, revealing that a classification based on underground
system characters does not show natural interspecific relationships and must be revised. This is also
apparent from trnL-F sequence analyses. Based on our results, the Praemorsa and Rhizomatosa
Groups can be merged.
Iran,ITS,phylogeny,Ranunculus,trnL-F
https://pbiosci.ut.ac.ir/article_22458.html
https://pbiosci.ut.ac.ir/article_22458_76245f10ff2e7164c31017d6ab6375a1.pdf
University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
DNA markers in hybrids of female Caspian kutum Rutilus
frisii kutum and male grass carp Ctenopharyngodon idella:
possible production of gynogenic progeny
49
102
EN
Mohammad Hassanzadeh
Saber
International Sturgeon Research Institute, P.O.-P.H.D
saber_merag6@yahoo.com
Shahrouz Baradaran
Noveiri
International Sturgeon Research Institute, P.O.-P.H.D
saber_merag5@yahoo.com
Mohammad
Pourkazemi
International Sturgeon Research Institute, P.O.-P.H.D
saber_merag4@yahoo.com
Mohammadreza
Nowruzfashkhami
International Sturgeon Research Institute, P.O.-P.H.D
saber_merag3@yahoo.com
Mahtab
Yarmohammadi
International Sturgeon Research Institute, P.O.-P.H.D
saber_merag2@yahoo.com
Alireza
Kashani Sabet
Fishes Rehabilitation and Breeding Center-P.H.D
saber_merag@yahoo.com
10.22059/pbs.2011.22459
Hybrids were produced by crossing female Caspian kutum Rutilus frisii kutum with male grass
carp Ctenopharyngodon idella. The genome of eight larvae and parents were studied using microsatellite
markers for genetic evaluation and verification. After DNA extraction from parent fish
and progeny, hybrid heritability of two loci was assessed using two pairs of microsatellite primers.
Hybridizied offspring showed as similar banding pattern to that of their maternal parent, without
heritability of the paternal genome. © 2011 Progress in Biological Sciences, Vol. 1, No.1, 49-54.Hybridizied offspring showed as similar banding pattern to that of their maternal parent, without
heritability of the paternal genome. © 2011 Progress in Biological Sciences, Vol. 1, No.1, 49-54
Grass carp,hybridization,Hybridizied,Kutum,microsatellite markers
https://pbiosci.ut.ac.ir/article_22459.html
https://pbiosci.ut.ac.ir/article_22459_4b131f5d28394ef7baf2ac6064823c07.pdf
University of Tehran Press
Progress in Biological Sciences
1016-1058
2228-7833
1
1
2011
06
01
AFLP reveals no sex-specific markers in Persian sturgeon
(Acipenser persicus) or beluga sturgeon (Huso huso) from
the southern Caspian Sea, Iran
55
114
EN
Mahtab
Yarmohammadi
Dr. Dadman International Sturgeon Research Institute
saber_merag2@yahoo.com
Mohammad
Pourkazemi1
Dr. Dadman International Sturgeon Research Institute
mahtabyarmohammadi5@gmail.com
Ahmad
Ghasemi
Center, Persian Gulf University
mahtabyarmohammadi4@gmail.com
Mohammad
Hassanzadeh
Dr. Dadman International Sturgeon Research Institute
mahtabyarmohammadi3@gmail.com
Fereidoon
Chakmehdouz
Dr. Dadman International Sturgeon Research Institute
mahtabyarmohammadi2@gmail.com
10.22059/pbs.2011.22460
The late sexual maturity in sturgeon and the absence of morphological differences between males
and females makes sex discrimination difficult. Identification of sex at an early life stage is of high
interest in caviar production because it allows efficient selection of females. In this study, the genome
of 10 mature male and 10 mature female specimens of Persian sturgeon (Acipenser persicus)
and beluga sturgeon (Huso huso) were screened using AFLP and 100 primer combinations. Results
showed a total of 3771 and 3779 scoreable bands in A. persicus and H. huso, respectively.
Approximately 30% of markers in A. persicus and 29.6% H. huso were polymorphic. No sex specific
makers were identified. The results of the present study suggest that the sex chromosomes are
not extensively differentiated in sturgeon species, or possibly the methods utilized were not sufficiently
sensitive to recognize them. © 2011 Progress in Biological Sciences, Vol 1, No.1, 55-60
Acipenser persicus,AFLP,Beluga,Huso huso,Persian Sturgeon,sex determination
https://pbiosci.ut.ac.ir/article_22460.html
https://pbiosci.ut.ac.ir/article_22460_e1500795fb507ed0a0a5174b3a03de78.pdf