@article { author = {Karimi, Reza and Norastehnia, Akbar and Abbaspour, Hossein and Saeidi Sar, Sakineh and Naeemi, Akram}, title = {Effects of copper oxide nanoparticles on the growth of Chlorella vulgaris}, journal = {Progress in Biological Sciences}, volume = {7}, number = {1}, pages = {11-20}, year = {2017}, publisher = {University of Tehran Press}, issn = {1016-1058}, eissn = {2228-7833}, doi = {10.22059/pbs.2018.226951.1253}, abstract = {The increase of copper oxide nanoparticle (CuO-NP) utilization in industry during recent years has resulted in their entry into aquatic ecosystems. In light of this fact, we have studied the toxicity of CuO-NPs at various concentrations on Chlorella vulgaris using an algal growth inhibition test (OECD201). Chlorella vulgaris was grown in positive Zander (Z-8 + N) media in a growth chamber. After reaching to the logarithmic growth phase, the algae were exposed to various concentrations of CuO-NPs at intervals of 24, 48 and 72 hours. Algal cell numbers were counted daily and the data were analyzed by Probit analysis. Some parameters such as: the effective concentration (EC10, EC50, EC90), no observed effect concentration (NOEC), specific growth rate (μ), doubling time (G) and percentage growth inhibition (I %) were calculated. The values of EC10 = 12.588, EC50 = 43.699, EC90 = 152.019 and NOEC = 4.3699 mg/L were obtained after 72 hours. The results showed significant differences between control and treatments at specified intervals of cell density and percentage of growth inhibition (P <0.05). In addition, chlorophyll and carotenoid content in treated cells were significantly lower compared to those in control samples; a decrease in the efficiency of photosynthesis is very probably a major mechanism in the reduced growth and viability of C. vulgaris exposed to CuO-NPs.}, keywords = {Toxicity of nanoparticles,Percent inhibition of growth,Specific growth rate,photosynthetic pigments}, url = {https://pbiosci.ut.ac.ir/article_69513.html}, eprint = {https://pbiosci.ut.ac.ir/article_69513_de3580ca9537871f3265f78d1941b676.pdf} }